RUNX1: Core binding factor (CBF) is a heterodimeric transcription factor that binds to the core element of many enhancers and promoters. The protein encoded by this gene represents the alpha subunit of CBF and is thought to be involved in the development of normal hematopoiesis. Chromosomal translocations involving this gene are well-documented and have been associated with several types of leukemia. Three transcript variants encoding different isoforms have been found for this gene.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Flow cytometric analysis of RUNX1/RUNX2/RUNX3 expression in HT-1080 cells using RUNX1/RUNX2/RUNX3 antibody . Green, isotype control; red, RUNX1/RUNX2/RUNX3.

Immunocytochemical staining of HT-1080 cells with RUNX1/RUNX2/RUNX3 antibody (Cat #61851, 1:1,000) . Nuclei were stained blue with DAPI; RUNX1/RUNX2/RUNX3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using RUNX1/RUNX2/RUNX3 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RUNX1/RUNX2/RUNX3 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using RUNX1/RUNX2/RUNX3 antibody . RUNX1/RUNX2/RUNX3 expression in wild type (WT) and RUNX1/RUNX2/RUNX3 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with RUNX1/RUNX2/RUNX3 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

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