The product of this gene is an enzyme involved in lipid metabolism, and it encodes cytosolic acetoacetyl-CoA thiolase. This gene shows complementary overlapping with the 3-prime region of the TCP1 gene in both mouse and human. These genes are encoded on opposite strands of DNA, as well as in opposite transcriptional orientation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

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Flow cytometric analysis of Acetyl-CoA acetyltransferase 2 expression in C2C12 cells using Acetyl-CoA acetyltransferase 2 antibody . Green, isotype control; red, Acetyl-CoA acetyltransferase 2.

Immunocytochemical staining of HepG2 cells with Acetyl-CoA acetyltransferase 2 antibody . Nuclei were stained blue with DAPI; Acetyl-CoA acetyltransferase 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using Acetyl-CoA acetyltransferase 2 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Acetyl-CoA acetyltransferase 2 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using Acetyl-CoA acetyltransferase 2 antibody . Acetyl-CoA acetyltransferase 2 expression in wild type (WT) and Acetyl-CoA acetyltransferase 2 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Acetyl-CoA acetyltransferase 2 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Validation of Acetyl-CoA acetyltransferase 2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Acetyl-CoA acetyltransferase 2 antibody and analyzed using CytoFLEX.

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