The protein encoded by this gene, which can bind actin, calmodulin, troponin C, and tropomyosin, may function in the structural organization of actin filaments. The encoded protein could play a role in smooth muscle contraction and cell adhesion. Several pseudogenes of this gene have been identified, and are present on chromosomes 1, 2, 3, 6, 9, 11, 13, 15, 16, 21 and 22. Alternative splicing results in multiple transcript variants encoding different isoforms.

推荐稀释比 WB: 1/500-1/2500; FC: 1/100-1/1000; ICC/IF: 1/100-1/1000

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Flow cytometric analysis of Calponin 2 expression in HT-1080 cells using Calponin 2 antibody . Green, isotype control; red, Calponin 2.

Immunocytochemical staining of HT-1080 cells with Calponin 2 associated protein antibody . Nuclei were stained blue with DAPI; Calponin 2 associated protein was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using calponin 2 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with calponin 2 antibody and HRP-conjugated goat anti-mouse secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using calponin 2 antibody . Calponin 2 expression in wild type (WT) and calponin 2 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with calponin 2 antibody and HRP-conjugated goat anti-mouse secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

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