This gene encodes a member of the CRMP (collapsing response mediator protein) family thought to be involved in neural development. Antibodies to the encoded protein were found in some patients with neurologic symptoms who had paraneoplastic syndrome. A pseudogene of this gene is found on chromosome 11. Multiple alternatively spliced variants, encoding the same protein, have been identified.

推荐稀释比 WB: 1/500-1/2500; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Flow cytometric analysis of Dihydropyrimidinase like 5 expression in HAP-1 cells using Dihydropyrimidinase like 5 antibody . Green, isotype control; red, Dihydropyrimidinase like 5.

Immunocytochemical staining of HAP-1 cells with Dihydropyrimidinase like 5 antibody . Nuclei were stained blue with DAPI; Dihydropyrimidinase like 5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using dihydropyrimidinase like 5 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with dihydropyrimidinase like 5 antibody and HRP-conjugated goat anti-mouse secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Western blotting analysis using dihydropyrimidinase like 5 antibody . Dihydropyrimidinase like 5 expression in wild-type (WT) and dihydropyrimidinase like 5 (DPYSL5) shRNA knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with dihydropyrimidinase like 5 antibody and HRP-conjugated goat anti-mouse secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Validation of Dihydropyrimidinase like 5 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HSHC cells were stained with Dihydropyrimidinase like 5 antibody and analyzed using BD flow cytometer.

Immunocytochemical staining of HAP-1 cells using Dihydropyrimidinase like 5 antibody , Top panel: wild-type (WT); Bottom panal: Dihydropyrimidinase like 5 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Dihydropyrimidinase like 5 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.

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