This gene is a member of the FAD-binding oxidoreductase/transferase type 4 family. It encodes a protein that catalyzes the second step of ether lipid biosynthesis in which acyl-dihydroxyacetonephosphate (DHAP) is converted to alkyl-DHAP by the addition of a long chain alcohol and the removal of a long-chain acid anion. The protein is localized to the inner aspect of the peroxisomal membrane and requires FAD as a cofactor. Mutations in this gene have been associated with rhizomelic chondrodysplasia punctata, type 3 and Zellweger syndrome.

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Immunocytochemical staining of HepG2 cells using Alkylglycerone phosphate synthase antibody , Top panel: wild-type (WT); Bottom panal: Alkylglycerone phosphate synthase shRNA knockdown (KD). Nuclei were stained blue with DAPI; Alkylglycerone phosphate synthase was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.

Validation of Alkylglycerone phosphate synthase knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HepG2 cells were stained with Alkylglycerone phosphate synthase antibody and analyzed using BD flow cytometer.

Flow cytometric analysis of Alkylglycerone phosphate synthase expression in HeLa cells using Alkylglycerone phosphate synthase antibody . Green, isotype control; red, Alkylglycerone phosphate synthase.

Immunocytochemical staining of Hela cells with Alkylglycerone phosphate synthase antibody . Nuclei were stained blue with DAPI; Alkylglycerone phosphate synthase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.

Western blotting analysis using alkylglycerone phosphate synthase antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with alkylglycerone phosphate synthase antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using alkylglycerone phosphate synthase antibody . Alkylglycerone phosphate synthase expression in wild-type (WT) and alkylglycerone phosphate synthase (AGPS) shRNA knockdown (KD) HepG2 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with alkylglycerone phosphate synthase antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

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