PRKCD: The protein encoded by this gene is a member of the protein kinase C family of serine- and threonine-specific protein kinases. The encoded protein is activated by diacylglycerol and is both a tumor suppressor and a positive regulator of cell cycle progression. Also, this protein can positively or negatively regulate apoptosis. Defects in this gene are a cause of autoimmune lymphoproliferative syndrome.

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Flow cytometric analysis of PKC delta + PKC theta expression in HT-1080 cells using PKC delta + PKC theta antibody . Green, isotype control; red, PKC delta + PKC theta.

Immunocytochemical staining of HT-1080 cells with PKC delta + PKC theta antibody . Nuclei were stained blue with DAPI; PKC delta + PKC theta was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using PKC delta + PKC theta antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PKC delta + PKC theta antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using PKC delta + PKC theta antibody . PKC delta + PKC theta expression in wild type (WT) and PKC delta + PKC theta shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with PKC delta + PKC theta antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

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