This gene encodes a G protein-coupled receptor (GPCR) that belongs to the oxoglutarate receptor family within the GPCR superfamily. The encoded protein is activated by the citric acid intermediate, oxoglutarate, as well as several cysteinyl leukotrienes, including leukotrienes E4, C4 and D4, which are implicated in many inflammatory disorders. In mice, a knock-out of this gene leads to middle ear inflammation, changes in the mucosal epithelium, and an increase in fluid behind the eardrum, and is associated with hearing loss. Alternative splicing results in multiple transcript variants.

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Immunocytochemical staining of HeLa cells using Oxoglutarate receptor 1 antibody , Top panel: wild-type (WT); Bottom panal: Oxoglutarate receptor 1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Oxoglutarate receptor 1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.

Flow cytometric analysis of Oxoglutarate receptor 1 expression in H9c2 cells using Oxoglutarate receptor 1 antibody . Green, isotype control; red, Oxoglutarate receptor 1.

Immunocytochemical staining of H9C2 cells with Oxoglutarate receptor 1 antibody . Nuclei were stained blue with DAPI; Oxoglutarate receptor 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.

Western blotting analysis using Oxoglutarate receptor 1 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Oxoglutarate receptor 1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using Oxoglutarate receptor 1 antibody . Oxoglutarate receptor 1 expression in wild type (WT) and Oxoglutarate receptor 1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with Oxoglutarate receptor 1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

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