This gene encodes an enzyme that plays a major role in polyamine metabolism and is important for the salvage pathway of both adenine and methionine. The encoded enzyme is deficient in many cancers. Multiple alternatively spliced transcript variants have been described for this gene.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

本产品仅用作科学研究！

Flow cytometric analysis of Methylthioadenosine phosphorylase expression in HT-1080 cells using Methylthioadenosine phosphorylase antibody . Green, isotype control; red, Methylthioadenosine phosphorylase.

Immunocytochemical staining of HT-1080 cells with Methylthioadenosine phosphorylase antibody . Nuclei were stained blue with DAPI; Methylthioadenosine phosphorylase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using Methylthioadenosine phosphorylase antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Methylthioadenosine phosphorylase antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using Methylthioadenosine phosphorylase antibody . Methylthioadenosine phosphorylase expression in wild type (WT) and Methylthioadenosine phosphorylase shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with Methylthioadenosine phosphorylase antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Store at -20℃ for one year.