Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000; IHC-P: 1/100-1/200

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Flow cytometric analysis of Histone H3 (acetyl K18) expression in HAP-1 cells using Histone H3 (acetyl K18) antibody . Green, isotype control; red, Histone H3 (acetyl K18).

Immunocytochemical staining of HAP-1 cells with Histone H3 (acetyl K18) antibody (Cat #61788, 1:1,000) . Nuclei were stained blue with DAPI; Histone H3 (acetyl K18) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.

Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using histone H3 (acetyl K19) antibody . Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.

Western blotting analysis using Histone H3 (acetyl K18) antibody . Total cell lysates (5 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Histone H3 (acetyl K18) antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using Histone H3 (acetyl K18) antibody . Histone H3 (acetyl K18) expression in wild type (WT) and Histone H3 (acetyl K18) shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Histone H3 (acetyl K18) antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Validation of Histone H3 (acetyl K18) knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Histone H3 (acetyl K18) antibody and analyzed using BD flow cytometer.

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