This gene encodes an enzyme responsible for the catalytic conversion of UTP (uridine triphosphate) to CTP (cytidine triphospate). This reaction is an important step in the biosynthesis of phospholipids and nucleic acids. Activity of this proten is important in the immune system, and loss of function of this gene has been associated with immunodeficiency. Alternative splicing results in multiple transcript variants.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Flow cytometric analysis of CTP synthase 1 expression in HepG2 cells using CTP synthase 1 antibody . Green, isotype control; red, CTP synthase 1.

Immunocytochemical staining of HT-1080 cells with CTP synthase 1 antibody . Nuclei were stained blue with DAPI; CTP synthase 1 with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using CTP synthase 1 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CTP synthase 1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using CTP synthase 1 antibody . CTP synthase 1 expression in wild type (WT) and CTP synthase 1 (CTPS1) shRNA knockdown (KD) 293T cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with CTP synthase 1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Immunocytochemical staining of 293T cells using CTP synthase 1 antibody , Top panel: wild-type (WT); Bottom panal: CTP synthase 1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; CTP synthase 1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.

Validation of CTP synthase 1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) 293T cells were stained with CTP synthase 1 antibody and analyzed using BD flow cytometer.

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