Enables several functions, including BAT3 complex binding activity; peptidase activity; and proteasome binding activity. Involved in several processes, including maintenance of unfolded protein; regulation of DNA-templated transcription; and regulation of catabolic process. Acts upstream of or within protein deubiquitination and protein stabilization. Predicted to be located in nucleoplasm. Predicted to be active in cytosol and nucleus.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Flow cytometric analysis of Ubiquitin specific peptidase 13 expression in HepG2 cells using Ubiquitin specific peptidase 13 antibody . Green, isotype control; red, Ubiquitin specific peptidase 13.

Immunocytochemical staining of HepG2 cells with Ubiquitin specific peptidase 13 antibody . Nuclei were stained blue with DAPI; Ubiquitin specific peptidase 13 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using Ubiquitin specific peptidase 13 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Ubiquitin specific peptidase 13 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using ubiquitin specific peptidase 13 antibody . Ubiquitin specific peptidase 13 expression in wild type (WT) and ubiquitin specific peptidase 13 (USP13) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ubiquitin specific peptidase 13 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

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