Enables ATP binding activity and dTMP kinase activity. Involved in dTDP biosynthetic process and thymidine biosynthetic process. Located in mitochondrion.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Immunocytochemical staining of HeLa cells using DTYMK antibody , Top panel: wild-type (WT); Bottom panal: DTYMK shRNA knockdown (KD). Nuclei were stained blue with DAPI; DTYMK was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.

Flow cytometric analysis of DTYMK expression in HeLa cells using DTYMK antibody . Green, isotype control; red, DTYMK.

Immunocytochemical staining of HeLa cells with DTYMK antibody . Nuclei were stained blue with DAPI; DTYMK was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using DTYMK antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DTYMK antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using DTYMK antibody . DTYMK expression in wild type (WT) and DTYMK shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with DTYMK antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Validation of DTYMK knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with DTYMK antibody and analyzed using BD flow cytometer.

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