This gene encodes an multipass transmembrane protein that functions as a key metabolic enzyme. The encoded protein catalyzes the conversion of diacylglycerol and fatty acyl CoA to triacylglycerol. This enzyme can also transfer acyl CoA to retinol. Activity of this protein may be associated with obesity and other metabolic diseases.

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Flow cytometric analysis of Diacylglycerol O-acyltransferase 1 expression in HeLa cells using Diacylglycerol O-acyltransferase 1 antibody . Green, isotype control; red, Diacylglycerol O-acyltransferase 1.

Immunocytochemical staining of Hela cells with Diacylglycerol O-acyltransferase 1 antibody (Cat #61926, 1:1,000). Nuclei were stained blue with DAPI; Diacylglycerol O-acyltransferase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using Diacylglycerol O-acyltransferase 1 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Diacylglycerol O-acyltransferase 1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using Diacylglycerol O-acyltransferase 1 antibody . Diacylglycerol O-acyltransferase 1 expression in wild type (WT) and Diacylglycerol O-acyltransferase 1 shRNA knockdown (KD) HT-1080 cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Diacylglycerol O-acyltransferase 1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

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