This gene encodes a eukaryotic translation initiation factor that catalyzes the formation of puromycin-sensitive 80 S preinitiation complexes and the poly(U)-directed synthesis of polyphenylalanine at low concentrations of Mg2+. This gene should not be confused with eIF2-alpha (EIF2S1, Gene ID: 1965), the alpha subunit of the eIF2 translation initiation complex. Although both of these proteins function in binding initiator tRNA to the 40 S ribosomal subunit, the encoded protein does so in a codon-dependent manner, whereas eIF2 complex requires GTP. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Validation of Eukaryotic translation initiation factor 2A knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HepG2 cells were stained with Eukaryotic translation initiation factor 2A antibody and analyzed using BD flow cytometer.

Flow cytometric analysis of Eukaryotic translation initiation factor 2A expression in HepG2 cells using Eukaryotic translation initiation factor 2A antibody . Green, isotype control; red, Eukaryotic translation initiation factor 2A.

Immunocytochemical staining of HT-1080 cells with Eukaryotic translation initiation factor 2A antibody . Nuclei were stained blue with DAPI; Eukaryotic translation initiation factor 2A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Immunocytochemical staining of HeLa cells using Eukaryotic translation initiation factor 2A antibody , Top panel: wild-type (WT); Bottom panal: Eukaryotic translation initiation factor 2A shRNA knockdown (KD). Nuclei were stained blue with DAPI; Acyl-CoA dehydrogenase short chain was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.

Western blotting analysis using eukaryotic translation initiation factor 2A antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with eukaryotic translation initiation factor 2A antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Western blotting analysis using eukaryotic translation initiation factor 2A antibody . Eukaryotic translation initiation factor 2A expression in wild-type (WT) and eukaryotic translation initiation factor 2A (EIF2A) shRNA knockdown (KD) HepG2 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with eukaryotic translation initiation factor 2A antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

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