The protein encoded by this gene is a nucleolar, zinc finger protein that preferentially binds to double-stranded (ds) RNA or RNA/DNA hybrids, rather than DNA alone. Mutational studies indicate that the zinc finger domains are not only essential for dsRNA binding, but are also required for its nucleolar localization. The encoded protein may be involved in cell growth and survival. It plays a role in protecting neurons by inhibiting cell cycle re-entry via stimulation of p21 gene expression. Alternative splicing of this gene results in multiple transcript variants.

推荐稀释比 WB: 1/500-1/2500; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

本产品仅用作科学研究！

Flow cytometric analysis of ZNF346 expression in HeLa cells using ZNF346 antibody . Green, isotype control; red, ZNF346.

Immunocytochemical staining of Hela cells with ZNF346 antibody . Nuclei were stained blue with DAPI; ZNF346 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using ZNF346 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ZNF346 antibody and HRP-conjugated goat anti-mouse secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Western blotting analysis using ZNF346 antibody . ZNF346 expression in wild-type (WT) and ZNF346 knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ZNF346 antibody and HRP-conjugated goat anti-mouse secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Store at -20℃ for one year.