This gene encodes a member of the tubulin superfamily. The encoded protein localizes to the centrosome where it binds to microtubules as part of a complex referred to as the gamma-tubulin ring complex. The protein mediates microtubule nucleation and is required for microtubule formation and progression of the cell cycle. A pseudogene of this gene is found on chromosome 7.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Flow cytometric analysis of gamma Tubulin expression in HepG2 cells using gamma Tubulin antibody . Green, isotype control; red, gamma Tubulin.

Immunocytochemical staining of HepG2 cells with Gamma Tubulin antibody (Cat #61875, 1:1,000) . Nuclei were stained blue with DAPI; Gamma Tubulin was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using gamma Tubulin antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with gamma Tubulin antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using gamma Tubulin antibody . gamma Tubulin expression in wild type (WT) and gamma Tubulin shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with gamma Tubulin antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Immunocytochemical staining of HeLa cells using gamma Tubulin antibody , Top panel: wild-type (WT); Bottom panal: gamma Tubulin shRNA knockdown (KD). Nuclei were stained blue with DAPI; gamma Tubulin was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.

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