This gene encodes a nuclear factor that is involved in the maintenance of pluripotency in stem cells and essential for embryogenesis. The encoded protein has a scaffold-attachment factor A/B, acinus and PIAS (SAP) domain that binds DNA and is thought to modify chromatin. Mice with a homozygous knockout of the orthologous gene die during late embryonic development or within hours after birth. Knockout embryos are normal in size at embryonic day 18.5 but exhibit skeletal and lung tissue abnormalities. This gene, when mutated, is highly expressed in embryonal carcinomas, pluripotent germ cell tumors, and other cancers and is thought to play an important role in tumor progression. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants.

推荐稀释比 WB: 1/500-1/2500; FC: 1/100-1/1000; ICC/IF: 1/100-1/1000

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Flow cytometric analysis of Developmental pluripotency associated 4 expression in HAP-1 cells using Developmental pluripotency associated 4 antibody . Green, isotype control; red, Developmental pluripotency associated 4.

Immunocytochemical staining of HAP-1 cells with Developmental pluripotency associated 4 antibody . Nuclei were stained blue with DAPI; Developmental pluripotency associated 4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using developmental pluripotency associated 4 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with developmental pluripotency associated 4 antibody and HRP-conjugated goat anti-mouse secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using developmental pluripotency associated 4 antibody . Developmental pluripotency associated 4 expression in wild-type (WT) and developmental pluripotency associated 4 (DPPA4) knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with developmental pluripotency associated 4 antibody and HRP-conjugated goat anti-mouse secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

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