Predicted to enable RNA polymerase II general transcription initiation factor activity. Involved in positive regulation of transcription by RNA polymerase II; transcription elongation by RNA polymerase II; and transcription initiation at RNA polymerase II promoter. Located in microtubule cytoskeleton and nucleoplasm. Part of transcription factor TFIIF complex.

推荐稀释比 WB: 1/1000-1/5000; ICC/IF: 1/100-1/1000

本产品仅用作科学研究！

Immunocytochemical staining of HeLa cells using GTF2F2 antibody , Top panel: wild-type (WT); Bottom panal: GTF2F2 shRNA knockdown (KD). Nuclei were stained blue with DAPI;GTF2F2 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.

Immunocytochemical staining of HepG2 cells with GTF2F2 antibody . Nuclei were stained blue with DAPI; GTF2F2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.

Western blotting analysis using GTF2F2 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with GTF2F2 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Western blotting analysis using GTF2F2 antibody . GTF2F2 expression in wild type (WT) and GTF2F2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with GTF2F2 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Store at -20℃ for one year.