This gene encodes the catalytic subunit of the DNA-dependent protein kinase (DNA-PK). It functions with the Ku70/Ku80 heterodimer protein in DNA double strand break repair and recombination. The protein encoded is a member of the PI3/PI4-kinase family.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Western blotting analysis using DNA PKcs antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DNA PKcs antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using DNA PKcs antibody . DNA PKcs expression in wild type (WT) and PRKDC shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with DNA PKcs antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit . PRKDC, DNA-dependent protein kinase catalytic subunit.

Validation of DNA PKcs knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with DNA PKcs antibody and analyzed using BD flow cytometer.

Immunocytochemical staining of Hela cells using DNA PKcs antibody , Top panel: wild-type (WT); Bottom panal: DNA PKcs shRNA knockdown (KD). Nuclei were stained blue with DAPI; DNA PKcs was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.

Flow cytometric analysis of DNA PKcs expression in HepG2 cells using DNA PKcs antibody . Green, isotype control; red, DNA PKcs.

Immunocytochemical staining of HepG2 cells with DNA PKcs antibody . Nuclei were stained blue with DAPI; DNA PKcs was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

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