Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD9 gene is located within the Williams syndrome common deletion region of chromosome 7, and heterozygous deletion of the FZD9 gene may contribute to the Williams syndrome phenotype. FZD9 is expressed predominantly in brain, testis, eye, skeletal muscle, and kidney.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000

本产品仅用作科学研究！

Validation of Frizzled class receptor 9 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Frizzled class receptor 9 antibody and analyzed using BD flow cytometer.

Western blotting analysis using frizzled class receptor 9 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with frizzled class receptor 9 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using frizzled class receptor 9 antibody . Frizzled class receptor 9 expression in wild type (WT) and frizzled class receptor 9 (FZD9) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with frizzled class receptor 9 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Store at -20℃ for one year.