Predicted to enable ATP hydrolysis activity; heat shock protein binding activity; and protein folding chaperone. Predicted to be involved in chaperone cofactor-dependent protein refolding and protein refolding. Located in cytosol and ribosome.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Immunocytochemical staining of HSHC cells using HSPA14 antibody , Top panel: wild-type (WT); Bottom panal: HSPA14 shRNA knockdown (KD). Nuclei were stained blue with DAPI; HSPA14 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.

Flow cytometric analysis of HSPA14 expression in HAP-1 cells using HSPA14 antibody . Green, isotype control; red, HSPA14.

Western blotting analysis using HSPA14 antibody . Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HSPA14 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Western blotting analysis using HSPA14 antibody . HSPA14 expression in wild-type (WT) and HSPA14 shRNA knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with HSPA14 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Validation of HSPA14 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HSHC cells were stained with HSPA14 antibody and analyzed using BD flow cytometer.

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