This gene encodes a member of the IQGAP family. The encoded protein contains three IQ domains, one calponin homology domain, one Ras-GAP domain and one WW domain. This protein interacts with components of the cytoskeleton, with cell adhesion molecules, and with several signaling molecules to regulate cell morphology and motility. It also acts as a tumor suppressor and has been found to play a role in regulating innate antiviral responses. Alternative splicing results in multiple transcript variants.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Immunocytochemical staining of Hela cells with IQGAP2 antibody . Nuclei were stained blue with DAPI; IQGAP2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Very low. Scale bar, 20 μm.

Western blotting analysis using IQGAP2 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with IQGAP2 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using IQGAP2 antibody . IQGAP2 expression in wild type (WT) and IQGAP2 shRNA knockdown (KD) 293T cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with IQGAP2 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Validation of IQGAP2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) 293T cells were stained with IQGAP2 antibody and analyzed using BD flow cytometer.

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