Enables RNA polymerase binding activity and histone binding activity. Involved in several processes, including negative regulation of apoptotic process; nucleosome assembly; and positive regulation of protein export from nucleus. Located in cytoplasm; nucleolus; and nucleoplasm.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Flow cytometric analysis of ANP32B expression in HepG2 cells using ANP32B antibody . Green, isotype control; red, ANP32B.

Immunocytochemical staining of HepG2 cells with ANP32B antibody . Nuclei were stained blue with DAPI; ANP32B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.

Western blotting analysis using ANP32B antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ANP32B antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit . ANP32B, acidic nuclear phosphoprotein 32 family member B.

Western blotting analysis using ANP32B antibody . ANP32B expression in wild type (WT) and ANP32B shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ANP32B antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

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