Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class I of the histone deacetylase family. It catalyzes the deacetylation of lysine residues in the histone N-terminal tails and represses transcription in large multiprotein complexes with transcriptional co-repressors. Multiple transcript variants encoding different isoforms have been found for this gene.

推荐稀释比 WB: 1/4000-1/20000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Flow cytometric analysis of Histone deacetylase 8 expression in HeLa cells using Histone deacetylase 8 antibody . Green, isotype control; red, Histone deacetylase 8.

Immunocytochemical staining of HeLa cells with Histone deacetylase 8 antibody . Nuclei were stained blue with DAPI; Histone deacetylase 8 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using histone deacetylase 8 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with histone deacetylase 8 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Western blotting analysis using histone deacetylase 8 antibody . Histone deacetylase 8 expression in wild-type (WT) and histone deacetylase 8 (HDAC8) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with histone deacetylase 8 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Validation of Histone deacetylase 8 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Histone deacetylase 8 antibody and analyzed using BD flow cytometer.

Immunocytochemical staining of HeLa cells using Histone deacetylase 8 antibody , Top panel: wild-type (WT); Bottom panal: Histone deacetylase 8 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Acyl-CoA dehydrogenase short chain was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.

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