This gene is a homolog of the Drosophila diaphanous gene, and has been linked to autosomal dominant, fully penetrant, nonsyndromic sensorineural progressive low-frequency hearing loss. Actin polymerization involves proteins known to interact with diaphanous protein in Drosophila and mouse. It has therefore been speculated that this gene may have a role in the regulation of actin polymerization in hair cells of the inner ear. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Western blotting analysis using Diaphanous related formin 1 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Diaphanous related formin 1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using Diaphanous related formin 1 antibody . Diaphanous related formin 1 expression in wild type (WT) and diaphanous related formin 1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Diaphanous related formin 1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Immunocytochemical staining of HepG2 cells with Diaphanous related formin 1 antibody . Nuclei were stained blue with DAPI; Diaphanous related formin 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Flow cytometric analysis of Diaphanous related formin 1 expression in HepG2 cells using Diaphanous related formin 1 antibody . Green, isotype control; red, Diaphanous related formin 1.

Validation of Diaphanous related formin 1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Diaphanous related formin 1 antibody and analyzed using BD flow cytometer.

Immunocytochemical staining of HeLa cells using Diaphanous related formin 1 antibody , Top panel: wild-type (WT); Bottom panal: Diaphanous related formin 1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Diaphanous related formin 1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.

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