The protein encoded by this gene belongs to the superfamily of small heat-shock proteins containing a conservative alpha-crystallin domain at the C-terminal part of the molecule. The expression of this gene in induced by estrogen in estrogen receptor-positive breast cancer cells, and this protein also functions as a chaperone in association with Bag3, a stimulator of macroautophagy. Thus, this gene appears to be involved in regulation of cell proliferation, apoptosis, and carcinogenesis, and mutations in this gene have been associated with different neuromuscular diseases, including Charcot-Marie-Tooth disease.

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Flow cytometric analysis of Hsp22 expression in HepG2 cells using Hsp22 antibody . Green, isotype control; red, Hsp22.

Immunocytochemical staining of HepG2 cells with Hsp22 antibody . Nuclei were stained blue with DAPI; Hsp22 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using Hsp22 antibody . Total cell lysates (50 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Hsp22 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using Hsp22 antibody . Hsp22 expression in wild type (WT) and Hsp22 shRNA knockdown (KD) 293T cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with Hsp22 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

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