The protein encoded by this gene is a cyclosporine-binding protein and is mainly located within the endoplasmic reticulum. It is associated with the secretory pathway and released in biological fluids. This protein can bind to cells derived from T- and B-lymphocytes, and may regulate cyclosporine A-mediated immunosuppression. Variants have been identified in this protein that give rise to recessive forms of osteogenesis imperfecta.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Flow cytometric analysis of Peptidylprolyl isomerase B expression in H9c2 cells using Peptidylprolyl isomerase B antibody . Green, isotype control; red, Peptidylprolyl isomerase B.

Immunocytochemical staining of H9C2 cells with Peptidylprolyl isomerase B antibody (Cat #61118, 1:1,000) . Nuclei were stained blue with DAPI; Peptidylprolyl isomerase B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using Peptidylprolyl isomerase B antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Peptidylprolyl isomerase B antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using peptidylprolyl isomerase B antibody . Peptidylprolyl isomerase B expression in wild type (WT) and peptidylprolyl isomerase B (PPIB) shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with peptidylprolyl isomerase B antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

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