The protein encoded by this gene is one of several isozymes of carbonic anhydrase, which catalyzes reversible hydration of carbon dioxide. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Two transcript variants encoding different isoforms have been found for this gene.

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Flow cytometric analysis of Carbonic anhydrase II expression in HeLa cells using Carbonic anhydrase II antibody . Green, isotype control; red, Carbonic anhydrase II.

Immunocytochemical staining of Hela cells with Carbonic anhydrase II antibody . Nuclei were stained blue with DAPI; Carbonic anhydrase II was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using Carbonic anhydrase II antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Carbonic anhydrase II antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using Carbonic anhydrase II antibody . Carbonic anhydrase II expression in wild type (WT) and Carbonic anhydrase II shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with Carbonic anhydrase II antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

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