Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia. Several transcript variants encoding different isoforms have been found for this gene.

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Validation of Dihydrofolate reductase knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Dihydrofolate reductase antibody and analyzed using BD flow cytometer.

Flow cytometric analysis of Dihydrofolate reductase expression in HeLa cells using Dihydrofolate reductase antibody . Green, isotype control; red, Dihydrofolate reductase.

Immunocytochemical staining of Hela cells with Dihydrofolate reductase antibody . Nuclei were stained blue with DAPI; Dihydrofolate reductase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using Dihydrofolate reductase antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Dihydrofolate reductase antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using Dihydrofolate reductase antibody . Dihydrofolate reductase expression in wild type (WT) and Dihydrofolate reductase shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with Dihydrofolate reductase antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

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