The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier. Mutations in this gene are associated with colchicine resistance and Inflammatory bowel disease 13. Alternative splicing and the use of alternative promoters results in multiple transcript variants.

推荐稀释比 WB: 1/1000-1/5000; ICC/IF: 1/100-1/1000; IHC-P: 1/100-1/200

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Immunocytochemical staining of HepG2 cells with ABCB1 antibody . Nuclei were stained blue with DAPI; ABCB1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.

Immunohistochemistry was performed on paraffin-embedded mouse kidney using ABCB1 antibody . Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.

Immunohistochemistry was performed on paraffin-embedded mouse liver using ABCB1 antibody . Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.

Western blotting analysis using ABCB1 antibody . Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ABCB1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit . ABCB1, ATP binding cassette subfamily B member 1.

Western blotting analysis using ABCB1 antibody . ABCB1 expression in wild type (WT) and ABCB1 shRNA knockdown (KD) Hela cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ABCB1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

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