This gene encodes a member of the human ARF gene family, which is part of the RAS superfamily. The ARF genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The product of this gene is localized to the plasma membrane, and regulates vesicular trafficking, remodelling of membrane lipids, and signaling pathways that lead to actin remodeling. A pseudogene of this gene is located on chromosome 7.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Validation of ADP ribosylation factor 6 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with ADP ribosylation factor 6 antibody and analyzed using BD flow cytometer.

Flow cytometric analysis of ADP ribosylation factor 6 expression in HT-1080 cells using ADP ribosylation factor 6 antibody . Green, isotype control; red, ADP ribosylation factor 6.

Immunocytochemical staining of HT-1080 cells with ADP ribosylation factor 6 antibody . Nuclei were stained blue with DAPI; ADP ribosylation factor 6 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using ADP ribosylation factor 6 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ADP ribosylation factor 6 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using ADP ribosylation factor 6 antibody . ADP ribosylation factor 6 expression in wild type (WT) and ADP ribosylation factor 6 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with ADP ribosylation factor 6 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

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