分子生物学
IVD分子诊断
细胞培养与分析
蛋白研究
细胞因子
重组蛋白
抗体
高通量测序建库
病原检测UCF系列
生物医药
工具酶
抑制剂激活剂与常用试剂
仪器
耗材
Kinesin Family Member 11 Recombinant Rabbit mAb [KD验证]
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This gene encodes a motor protein that belongs to the kinesin-like protein family. Members of this protein family are known to be involved in various kinds of spindle dynamics. The function of this gene product includes chromosome positioning, centrosome separation and establishing a bipolar spindle during cell mitosis.
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推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000
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Western blotting analysis using kinesin family member 11 antibody . Kinesin family member 11 expression in wild type (WT) and kinesin family member 11 (KIF11) shRNA knockdown (KD) 293T cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with kinesin family member 11 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Flow cytometric analysis of Kinesin family member 11 expression in HAP-1 cells using Kinesin family member 11 antibody . Green, isotype control; red, Kinesin family member 11.

Immunocytochemical staining of HAP-1 cells with Kinesin family member 11 antibody . Nuclei were stained blue with DAPI; Kinesin family member 11 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using Kinesin family member 11 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Kinesin family member 11 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Immunocytochemical staining of 293T cells using Kinesin family member 11 antibody , Top panel: wild-type (WT); Bottom panal: Kinesin family member 11 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Kinesin family member 11 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.

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