Interaction of Fas ligand (TNFSF6) with the FAS antigen (TNFRSF6) mediates programmed cell death, also called apoptosis, in a number of organ systems. The protein encoded by this gene binds to FAS antigen and can initiate apoptosis or enhance apoptosis initiated through FAS antigen. Initiation of apoptosis by the protein encoded by this gene requires a ubiquitin-like domain but not the FAS-binding domain.

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Validation of Fas associated factor 1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Fas associated factor 1 antibody and analyzed using BD flow cytometer.

Flow cytometric analysis of Fas associated factor 1 expression in HepG2 cells using Fas associated factor 1 antibody . Green, isotype control; red, Fas associated factor 1.

Immunocytochemical staining of HepG2 cells with Fas associated factor 1 antibody . Nuclei were stained blue with DAPI; Fas associated factor 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using fas associated factor 1 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with fas associated factor 1 antibody and HRP-conjugated goat anti-mouse secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Western blotting analysis using fas associated factor 1 antibody . Fas associated factor 1 expression in wild type (WT) and fas associated factor 1 (FAF1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with fas associated factor 1 antibody and HRP-conjugated goat anti-mouse secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Immunocytochemical staining of HeLa cells using Fas associated factor 1 antibody , Top panel: wild-type (WT); Bottom panal: Fas associated factor 1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Fas associated factor 1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.

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