This gene encodes a cytokine receptor-like factor that may negatively regulate cell cycle progression at the G0/G1 phase. Studies of the related rat protein suggest that it may regulate neuronal morphology and synaptic vesicle biogenesis. This gene is one of several genes located in the neurofibromatosis type I tumor suppressor region on the q arm of chromosome 17, a region that is subject to microdeletions, duplications, chromosomal breaks and rearrangements. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 2 and 5.

推荐稀释比 WB: 1/200-1/1000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000; IHC-P: 1/100-1/200

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Immunohistochemistry was performed on paraffin-embedded human lymphoma using cytokine receptor like factor 3 antibody . Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.

Flow cytometric analysis of Cytokine receptor like factor 3 expression in HepG2 cells using Cytokine receptor like factor 3 antibody . Green, isotype control; red, Cytokine receptor like factor 3.

Immunocytochemical staining of HepG2 cells with Cytokine receptor like factor 3 antibody . Nuclei were stained blue with DAPI; Cytokine receptor like factor 3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using cytokine receptor like factor 3 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with cytokine receptor like factor 3 antibody and HRP-conjugated goat anti-mouse secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Western blotting analysis using cytokine receptor like factor 3 antibody . Cytokine receptor like factor 3 expression in wild-type (WT) and cytokine receptor like factor 3 (CRLF3) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with cytokine receptor like factor 3 antibody and HRP-conjugated goat anti-mouse secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Validation of Cytokine receptor like factor 3 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Cytokine receptor like factor 3 antibody and analyzed using BD flow cytometer.

Immunocytochemical staining of HeLa cells using Cytokine receptor like factor 3 antibody , Top panel: wild-type (WT); Bottom panal: Cytokine receptor like factor 3 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Cytokine receptor like factor 3 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.

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