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Flow cytometric analysis of BNIP3 expression in C2C12 cells using BNIP3 antibody . Green, isotype control; red, BNIP3.
Immunocytochemical staining of C2C12 cells with BNIP3 antibody . Nuclei were stained blue with DAPI; BNIP3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using BNIP3 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with BNIP3 antibody and HRP-conjugated goat anti-mouse secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .
Western blotting analysis using BNIP3 antibody . BNIP3 expression in wild-type (WT) and BNIP3 knockout (KO) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with BNIP3 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .
