The protein encoded by this gene is a ligand-activated helix-loop-helix transcription factor involved in the regulation of biological responses to planar aromatic hydrocarbons. This receptor has been shown to regulate xenobiotic-metabolizing enzymes such as cytochrome P450. Before ligand binding, the encoded protein is sequestered in the cytoplasm; upon ligand binding, this protein moves to the nucleus and stimulates transcription of target genes.

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Flow cytometric analysis of Aryl hydrocarbon receptor expression in HepG2 cells using Aryl hydrocarbon receptor antibody . Green, isotype control; red, Aryl hydrocarbon receptor.

Immunocytochemical staining of HepG2 cells with Aryl hydrocarbon receptor antibody . Nuclei were stained blue with DAPI; Aryl hydrocarbon receptor was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using Aryl hydrocarbon receptor antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Aryl hydrocarbon receptor antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using Aryl hydrocarbon receptor antibody . Aryl hydrocarbon receptor expression in wild type (WT) and aryl hydrocarbon receptor shRNA knockdown (KD) Hela cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with Aryl hydrocarbon receptor antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

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