Enables acetyltransferase activity and phosphorylase kinase regulator activity. Involved in regulation of transcription by RNA polymerase II and tRNA wobble uridine modification. Located in cytosol and nucleolus. Part of elongator holoenzyme complex.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Flow cytometric analysis of ELP3 expression in C2C12 cells using ELP3 antibody . Green, isotype control; red, ELP3.

Immunocytochemical staining of C2C12 cells with ELP3 antibody . Nuclei were stained blue with DAPI; ELP3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.

Western blotting analysis using ELP3 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ELP3 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Western blotting analysis using ELP3 antibody . ELP3 expression in wild-type (WT) and ELP3 shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ELP3 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Validation of ELP3 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HT-1080 cells were stained with ELP3 antibody and analyzed using BD flow cytometer.

Immunocytochemical staining of HT-1080 cells using ELP3 antibody , Top panel: wild-type (WT); Bottom panal: ELP3 shRNA knockdown (KD). Nuclei were stained blue with DAPI; ELP3 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.

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