CHMP5 belongs to the chromatin-modifying protein/charged multivesicular body protein (CHMP) family. These proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies (MVBs). Some CHMPs have both nuclear and cytoplasmic/vesicular distributions, and one such CHMP, CHMP1A (MIM 164010), is required for both MVB formation and regulation of cell cycle progression (Tsang et al., 2006

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Flow cytometric analysis of Charged multivesicular body protein 5 expression in HepG2 cells using Charged multivesicular body protein 5 antibody . Green, isotype control; red, Charged multivesicular body protein 5.

Western blotting analysis using charged multivesicular body protein 5 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with charged multivesicular body protein 5 antibody and HRP-conjugated goat anti-mouse secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Western blotting analysis using charged multivesicular body protein 5 antibody . Charged multivesicular body protein 5 expression in wild-type (WT) and charged multivesicular body protein 5 (CHMP5) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with charged multivesicular body protein 5 antibody and HRP-conjugated goat anti-mouse secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

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