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Flow cytometric analysis of Catenin beta 1 expression in HAP-1 cells using Catenin beta 1 antibody . Green, isotype control; red, Catenin beta 1.
Immunocytochemical staining of HAP-1 cells with Catenin beta 1 antibody . Nuclei were stained blue with DAPI; Catenin beta 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain: Medium. Scale bar, 20 μm.
Western blotting analysis using catenin beta 1 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with catenin beta 1 antibody and HRP-conjugated goat anti-mouse secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .
Western blotting analysis using catenin beta 1 antibody . Catenin beta 1 expression in wild-type (WT) and catenin beta 1 (CTNNB1) knockout (KO) 293T cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with catenin beta 1 antibody and HRP-conjugated goat anti-mouse secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .
Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using catenin beta 1 antibody . Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
