This gene encodes a member of the A-kinase anchor protein family. A-kinase anchor proteins are scaffold proteins that contain a binding domain for the RI/RII subunit of protein kinase A (PKA) and recruit PKA and other signaling molecules to specific subcellular locations. This gene encodes a nuclear A-kinase anchor protein that binds to the RII alpha subunit of PKA and may play a role in chromosome condensation during mitosis by targeting PKA and the condensin complex to chromatin. A pseudogene of this gene is located on the short arm of chromosome 9.

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Flow cytometric analysis of A-kinase anchoring protein 8 expression in C2C12 cells using A-kinase anchoring protein 8 antibody . Green, isotype control; red, A-kinase anchoring protein 8.

Immunocytochemical staining of C2C12 cells with A-kinase anchoring protein 8 antibody . Nuclei were stained blue with DAPI; A-kinase anchoring protein 8 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.

Western blotting analysis using A-kinase anchoring protein 8 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with A-kinase anchoring protein 8 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using A-kinase anchoring protein 8 antibody . A-kinase anchoring protein 8 expression in wild type (WT) and A-kinase anchoring protein 8 (AKAP8) shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with A-kinase anchoring protein 8 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

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