This gene encodes the cytosolic form of the enzyme branched-chain amino acid transaminase. This enzyme catalyzes the reversible transamination of branched-chain alpha-keto acids to branched-chain L-amino acids essential for cell growth. Two different clinical disorders have been attributed to a defect of branched-chain amino acid transamination: hypervalinemia and hyperleucine-isoleucinemia. As there is also a gene encoding a mitochondrial form of this enzyme, mutations in either gene may contribute to these disorders. Alternatively spliced transcript variants have been described.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/1/100-1000; ICC/IF: 1/100-1/1000; IHC-P: 1/100-1/200

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Western blotting analysis using BCAT1 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with BCAT1 antibody and HRP-conjugated goat anti-mouse secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using BCAT1 antibody . BCAT1 expression in wild type (WT) and BCAT1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with BCAT1 antibody and HRP-conjugated goat anti-mouse secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Flow cytometric analysis of BCAT1 expression in C2C12 cells using BCAT1 antibody . Green, isotype control; red, BCAT1.

Validation of BCAT1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with BCAT1 antibody and analyzed using BD flow cytometer.

Immunocytochemical staining of HeLa cells using BCAT1 antibody , Top panel: wild-type (WT); Bottom panal: BCAT1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; BCAT1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.

Immunohistochemistry was performed on paraffin-embedded human pancreatic adenocarcinoma using BCAT1 antibody . Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.

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