This gene encodes a protein that plays a critical role in dynein arm assembly and motile cilia function. Mutations in this gene result in primary ciliary dyskinesia. Naturally occuring readthrough transcription occurs from this locus to the downstream t-complex 10 like (TCP10L) gene.

推荐稀释比 WB: 1/500-1/2500; FC: 1/100-1/1000; ICC/IF: 1/100-1/1000

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Flow cytometric analysis of CFAP298 expression in HepG2 cells using CFAP298 antibody . Green, isotype control; red, CFAP298.

Immunocytochemical staining of HepG2 cells with CFAP298 antibody . Nuclei were stained blue with DAPI; CFAP298 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using CFAP298 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CFAP298 antibody and HRP-conjugated goat anti-mouse secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using CFAP298 antibody . CFAP298 expression in wild type (WT) and CFAP298 shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with CFAP298 antibody and HRP-conjugated goat anti-mouse secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

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