This gene encodes a downstream nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis. This gene is known to play a role in the development and progression of multiple cancers as a transcriptional regulator of this signaling pathway and may function as a potential target for cancer treatment. Alternative splicing results in multiple transcript variants encoding different isoforms.

推荐稀释比 WB: 1/4000-1/20000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Flow cytometric analysis of Phospho-YAP1 (S127) expression in HepG2 cells using Phospho-YAP1 (S127) antibody . Green, isotype control; red, Phospho-YAP1 (S127).

Immunocytochemical staining of HepG2 cells with Phospho-YAP1 (S127) antibody . Nuclei were stained blue with DAPI; Phospho-YAP1 (S127) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using phospho-YAP1 (S127) antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-YAP1 (S127) antibody and HRP-conjugated goat anti rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Western blotting analysis using phospho-YAP1 (S127) antibody . Phospho-YAP1 (S127) expression in wild type (WT) and YAP1 knockout (KO) HSHC cells with 40 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with phospho-YAP1 (S127) antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

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