This gene encodes a downstream nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis. This gene is known to play a role in the development and progression of multiple cancers as a transcriptional regulator of this signaling pathway and may function as a potential target for cancer treatment. Alternative splicing results in multiple transcript variants encoding different isoforms.

推荐稀释比 WB: 1/2000-1/10000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Flow cytometric analysis of YAP1 expression in HepG2 cells using YAP1 antibody . Green, isotype control; red, YAP1.

Immunocytochemical staining of HepG2 cells with YAP1 antibody . Nuclei were stained blue with DAPI; YAP1 was stained magenta with Alexa Fluor® 647. lmages were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using YAP1 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with YAP1 antibody and HRP-conjugated goat anti rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Western blotting analysis using YAP1 antibody . YAP1 expression in wild type (WT) and YAP1 knockout (KO) HSHC cells with 40 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with YAP1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

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