This gene encodes a protein that is a member of the SUMO (small ubiquitin-like modifier) protein family. It functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. It is not active until the last four amino acids of the carboxy-terminus have been cleaved off. Several pseudogenes have been reported for this gene. Alternate transcriptional splice variants encoding different isoforms have been characterized.

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Flow cytometric analysis of Small ubiquitin like modifier 1 expression in HAP-1 cells using Small ubiquitin like modifier 1 antibody . Green, isotype control; red, Small ubiquitin like modifier 1.

Western blotting analysis using small ubiquitin like modifier 1 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with small ubiquitin like modifier 1 antibody and HRP-conjugated goat anti-mouse secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using small ubiquitin like modifier 1 antibody . Small ubiquitin like modifier 1 expression in wild-type (WT) and small ubiquitin like modifier 1 (SUMO1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with small ubiquitin like modifier 1 antibody and HRP-conjugated goat anti-mouse secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Validation of Small ubiquitin like modifier 1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Small ubiquitin like modifier 1 antibody and analyzed using BD flow cytometer.

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