The protein encoded by this gene is an omega class glutathione S-transferase (GST) with glutathione-dependent thiol transferase and dehydroascorbate reductase activities. GSTs are involved in the metabolism of xenobiotics and carcinogens. The encoded protein acts as a homodimer and is found in the cytoplasm. Three transcript variants encoding different isoforms have been found for this gene.

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Flow cytometric analysis of Glutathione S-transferase omega 1 expression in HepG2 cells using Glutathione S-transferase omega 1 antibody . Green, isotype control; red, Glutathione S-transferase omega 1.

Immunocytochemical staining of HepG2 cells with Glutathione S-transferase omega 1 antibody . Nuclei were stained blue with DAPI; Glutathione S-transferase omega 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using glutathione S-transferase omega 1 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with glutathione S-transferase omega 1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Western blotting analysis using glutathione S-transferase omega 1 antibody . Glutathione S-transferase omega 1 expression in wild type (WT) and glutathione S-transferase omega 1 (GSTO1) knockout (KO) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with glutathione S-transferase omega 1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

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