The protein encoded by this gene is a zinc finger transcription factor that binds to GC-rich motifs of many promoters. The encoded protein is involved in many cellular processes, including cell differentiation, cell growth, apoptosis, immune responses, response to DNA damage, and chromatin remodeling. Post-translational modifications such as phosphorylation, acetylation, glycosylation, and proteolytic processing significantly affect the activity of this protein, which can be an activator or a repressor. Three transcript variants encoding different isoforms have been found for this gene.

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Flow cytometric analysis of SP1 expression in HAP-1 cells using SP1 antibody . Green, isotype control; red, SP1.

Immunocytochemical staining of HAP-1 cells with SP1 antibody . Nuclei were stained blue with DAPI; SP1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using SP1 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SP1 antibody and HRP-conjugated goat anti-mouse secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using SP1 antibody . SP1 expression in wild-type (WT) and SP1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with SP1 antibody and HRP-conjugated goat anti-mouse secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Validation of SP1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with SP1 antibody and analyzed using BD flow cytometer.

Immunocytochemical staining of HeLa cells using SP1 antibody , Top panel: wild-type (WT); Bottom panal: SP1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; SP1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.

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