This gene encodes an enzyme that catalyzes the NAD(P)H-dependent conversion of pyrroline-5-carboxylate to proline. This enzyme may also play a physiologic role in the generation of NADP(+) in some cell types. The protein forms a homopolymer and localizes to the mitochondrion. Alternative splicing results in multiple transcript variants.

推荐稀释比 WB: 1/500-1/2500; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Flow cytometric analysis of PYCR1 expression in HepG2 cells using PYCR1 antibody . Green, isotype control; red, PYCR1.

Western blotting analysis using PYCR1 antibody . PYCR1 expression in wild-type (WT) and PYCR1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with PYCR1 antibody and HRP-conjugated goat anti-mouse secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Western blotting analysis using PYCR1 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PYCR1 antibody and HRP-conjugated goat anti-mouse secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Validation of PYCR1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with PYCR1 antibody and analyzed using BD flow cytometer.

Immunocytochemical staining of HeLa cells using PYCR1 antibody , Top panel: wild-type (WT); Bottom panal: PYCR1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; PYCR1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.

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