The protein encoded by this gene is one subunit of a cleavage factor required for 3' RNA cleavage and polyadenylation processing. The interaction of the protein with the RNA is one of the earliest steps in the assembly of the 3' end processing complex and facilitates the recruitment of other processing factors. This gene encodes the 25kD subunit of the protein complex, which is composed of four polypeptides.

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Flow cytometric analysis of Nudix hydrolase 21 expression in HAP-1 cells using Nudix hydrolase 21 antibody . Green, isotype control; red, Nudix hydrolase 21.

Western blotting analysis using nudix hydrolase 21 antibody . Nudix hydrolase 21 expression in wild-type (WT) and nudix hydrolase 21 (NUDT21) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with nudix hydrolase 21 antibody and HRP-conjugated goat anti-mouse secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Western blotting analysis using nudix hydrolase 21 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with nudix hydrolase 21 antibody and HRP-conjugated goat anti-mouse secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Validation of Nudix hydrolase 21 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Nudix hydrolase 21 antibody and analyzed using BD flow cytometer.

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