The protein encoded by this gene is a G protein-coupled receptor which activates a phosphatidylinositol-calcium second messenger system. Its ligand, endothelin, consists of a family of three potent vasoactive peptides: ET1, ET2, and ET3. Studies suggest that the multigenic disorder, Hirschsprung disease type 2, is due to mutations in the endothelin receptor type B gene. Alternative splicing and the use of alternative promoters results in multiple transcript variants.

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Flow cytometric analysis of Endothelin receptor type B expression in HepG2 cells using Endothelin receptor type B antibody . Green, isotype control; red, Endothelin receptor type B.

Immunocytochemical staining of HepG2 cells with Endothelin receptor type B1 antibody . Nuclei were stained blue with DAPI; Endothelin receptor type B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.

Western blotting analysis using endothelin receptor type B antibody . Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with endothelin receptor type B antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Western blotting analysis using endothelin receptor type B antibody . Endothelin receptor type B expression in wild-type (WT) and endothelin receptor type B (EDNRB) knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with endothelin receptor type B antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

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